Coenzyme A Assay Kit
Coenzyme A Assay Kit
Coenzyme A (CoA) is one of the most important cofactors in metabolism. It participates in fatty acid synthesis and oxidation, the TCA cycle, amino acid metabolism, and hundreds of acyltransferase reactions. Dysregulated CoA metabolism has been linked to metabolic syndrome, neurodegeneration (including pantothenate kinase-associated neurodegeneration), and cancer.
CoA and its derivatives (acetyl-CoA, malonyl-CoA, succinyl-CoA) are often measured via LC-MS, which limits throughput and accessibility.
Our FRET-based assay directly measures CoA concentration in real time. No enzyme-coupled reactions, no mass spectrometry—just add your sample to a standard plate reader.
Applications
Our assays enable sensitive and reliable measurement of Coenzyme A in biological samples, supporting metabolic pathway analysis, mechanistic studies, and drug discovery research across diverse biological systems.
- Metabolic profiling and pathway analysis
- Drug metabolism and toxicology studies
- Enzyme activity assays (acyltransferases, CoA-dependent enzymes)
- Cancer metabolism research
- Neurodegenerative disease research
Specifications
Optimized for robustness, scalability, and integration into high-throughput screening workflows.
Format
96-well and 384-well compatible
Readout
FRET-based fluorescence, real-time kinetic
Detection
Direct measurement of CoA concentration
Sample types
Cell lysates, tissue homogenates, purified enzyme reactions
Sample types
Cell lysates, tissue homogenates, purified enzyme reactions
Equipment
Standard fluorescence plate reader
Availability
Contact us for availability and early access. Our team is happy to provide technical details, answer questions, and help you select the best solution for your research needs.