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Percepta Bioscience
Services

Custom assay development

If the molecule you need to measure has no good assay, we build one. Same FRET sensor platform, same optimisation loop, applied to your target rather than ours.

How an engagement runs

Four stages, with a decision point at each one.

Stage 01

Feasibility

We assess the target, the sample matrix and the concentration range you need to resolve, and tell you honestly whether a sensor is the right tool.

Stage 02

Sensor generation

Candidate sensors are generated against your molecule and triaged down to the constructs worth building.

Stage 03

Optimisation

High-throughput engineering tunes dynamic range, affinity, specificity against near neighbours, and expression. Optimised assays typically reach a Z′ factor above 0.8.

Stage 04

Transfer

Validation in your matrix, a written protocol, and the assay running on your plate reader with your team. Typical timeline from kickoff to transfer is two to four months.

What we're a good fit for

Enzymes whose product has no direct detection method and is currently inferred through a coupled cascade.

Metabolites currently measured by LC-MS where instrument time is capping library size.

Programmes that need initial rates and mechanism, not just endpoint inhibition.

Targets that have been shelved specifically because the assay was the blocker.

What you should tell us

TargetMolecule or enzyme product
MatrixBuffer, lysate, serum, media, tissue extract
RangeConcentrations you need to resolve
ThroughputPlates per week, plate format
ReaderInstrument and available filter sets
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