Orotate Assay Kit
A plate-based route to orotate for DHODH programmes — direct, kinetic, and free of the coupled-dye workaround.
The committed step of pyrimidine synthesis, read directly.
Dihydroorotate dehydrogenase oxidises dihydroorotate to orotate, the rate-limiting step of de novo pyrimidine synthesis. Blocking it starves proliferating lymphocytes and tumour cells of nucleotides — the mechanism behind teriflunomide in multiple sclerosis, and behind the next-generation DHODH inhibitors now in Phase 2/3.
The enzyme is usually followed indirectly, through its electron acceptor rather than its product, so anything in a library that reduces the dye reads as activity. Our sensor binds orotate itself, in real time.
DHODH inhibitor screening
Primary and confirmatory screens on a direct product readout rather than a coupled dye.
Antiproliferative pharmacology
Dose-response and time-course work on pyrimidine-pathway inhibition with a continuous readout.
Redox interference triage
Separate genuine inhibition from redox-cycling compounds that defeat dye-coupled formats.
Request information for the Orotate Assay Kit
Contact us for availability, pricing and early access. Our team is happy to provide technical details and help you select the right configuration for your work.